Biochemistry Acs Practice Exam

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    Omega-3 fatty acids have which of the following? A Nonenzymatic glycosylation or glycation creates glycoproteins by the chemical addition of sugars to polypeptides. Since this type of glycosylation is nonenzymatic, the time and the concentration of sugar control glycosylation. Because people with higher circulating levels of glucose have higher levels of nonenzymatic glycosylation, measurement of the glycosylated hemoglobin A1c is a diagnostic test used to monitor blood sugar levels in persons with diabetes.

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    C Ribose is a pentose, a simple sugar monosaccharide that has five carbon atoms per molecule. It is synthesized in the body and obtained in small amounts from consumption of ripe fruits and vegetables. Deoxyribose, a component of DNA, also is a pentose. D The optimum pH for enzymes varies for different enzymes and even enzymes with similar actions may have different optimal pH based on where they act. For example, trypsin, a digestive enzyme that acts in the small intestine has an optimal pH of 8 while pepsin, which acts in the more acidic milieu of the stomach, has an optimal pH of 2. B A catabolic enzyme engages in destructive metabolism, which involves degrading or breaking down complex molecules into simpler ones with the resulting release of energy. In the body, the breakdown of food in the gastrointestinal tract by a variety of digestive enzymes is an example of a catabolic process.

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    The opposite of catabolism is anabolism. C Anabolism is the opposite of catabolism and is a set of metabolic pathways that serve to create, construct, or synthesize larger molecules from smaller ones, such as the synthesis of carbohydrates, proteins and fatty acids. Anabolic processes consume or require energy rather than releasing energy. Examples of anabolic processes include gluconeogenesis, glyoxylate cycle, and glycosylation. C Allosteric enzymes change their configurations when they bind with cofactors. Their catalytic activity is altered — either enhanced or reduced — by binding of specific ligands at sites other than the substrate-binding site.

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    Peptidases are a subgroup of proteases that that catalyze the hydrolysis of peptide linkages; they are present in plants and yeast and in the body. In the body, they are involved in digestion. B Amylase is the class of enzymes involved in the breakdown and digestion of starches — complex carbohydrates — into simple sugars to supply energy. Amylases are glycoside hydrolases and act on? Plants and some bacteria make amylases. In the body, they are present in saliva and are produced by salivary glands and the pancreas. C The stable three-dimensional shape and orientation of a protein its unique folds, termed its native configuration determine its function and chemical reactivity. Proteins are composed of series of as many as twenty different L-? Proteins are distinguished by their configurations into three broad classes — globular proteins, fibrous proteins, and membrane proteins. D The biosynthesis of a protein molecule uses information encoded in genes.

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    The amino acid sequence of the protein is delineated by the nucleotide sequence of the gene that encodes the protein. Codons, sequences of three adjacent nucleotides along a DNA or messenger RNA molecule, designate the specific amino acid to be incorporated into a polypeptide. C Peptide bonds are the primary linkages of all proteins.

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    They are the chemical connections that form between the carboxyl group COOH of one amino acid and the amino group NH2 of adjacent amino acids. A dipeptide contains two amino acids, a tripeptide three, a tetrapeptide four, and so on. The products of protein catabolism are used to synthesize new amino acids or are converted to other compounds via the citric acid cycle. Protein catabolism is most often carried out by proteases. A The citric acid cycle, also known as the tricarboxylic acid cycle TCA cycle , the Krebs cycle, or the Szent-Gyorgyi-Krebs cycle is a key component of the metabolic pathways that chemically converts proteins, carbohydrates, and fats into usable energy.

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    It is called citric acid cycle because the citric acid is the first product and the final reactant of the metabolic pathway. D The substrate of the citric acid cycle is acetyl coenzyme A. It is derived from glycolysis by a decarboxylating dehydrogenase activity of pyruvate dehydrogenase. The cycle yields the following intermediates: citrate, cis-aconitate, iso-citrate, alpha-ketoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate. At the conclusion of each cycle, the four-carbon oxaloacetate has been regenerated, and the cycle begins again. D Adenosine triphosphate ATP is an energy-filled nucleotide that powers the cells and stores energy. It is present in all life forms, from simple, single-celled organisms to humans. Organisms catabolize carbohydrates to create ATP. Then, the ATP is used for anabolic cellular reactions.

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    Each molecule of glucose can be catabolized to as many as 34 molecules of ATP. B Oxidative phosphorylation is a biochemical process that takes place in cells at a plasma membrane. The final metabolic pathway of cellular respiration, it occurs after glycolysis and the citric acid cycle. As many as 26 of 30 ATP molecules generated from a single glucose molecule during cellular respiration result from the process of oxidative phosphorylation. C The reduced intermediaries of the citric acid cycle are the source for multiple biosynthetic pathways. Every round of the cycle joins an acetyl group to oxaloacetate, oxidizing two carbons off as Co2, which leaves succinate.

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    Succinate is converted back to oxaloacetate producing one GTP and reduced cofactors. The rate of the cycle is determined by the availability of substrates, inhibition of accumulating products and allosteric inhibition of enzymes. A Free or unesterified fatty acids are released by the hydrolysis of triglycerides within adipose tissue. Free fatty acids can be used as an immediate source of energy by multiple organs and tissue and can be converted by the liver into ketone bodies. Because free fatty acids can interact with a variety of enzyme systems, they must be rapidly sequestered in tissues to ensure that their activities are closely regulated.

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    C An ester is formed when an alcohol combines with an acid in a reaction to form an organic compound. While fatty acids occur in nature in their free unesterified state, they are most often found as esters linked to glycerol, cholesterol, or long-chain aliphatic alcohols and as amides in sphingolipids. C Omega-3 fatty acids have a final carbon-carbon double bond in the third bond from the methyl end of the fatty acid. Omega-3 fatty acids — eicosapentaenoic acid and docosahexaenoic acid may be derived from the diet. They are present in oily or fatty fish, such as salmon, tuna, sardines, trout, and mackerel, and alpha linolenic acid is derived from plants.

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